Contents ⇒

Bioprocess design/Biofuel production/Bioremediation/Probiotics development/Functional food production

 

Production of useful lipids by filamentous fungi

We have found that mycelia of the fungus Mortierella alpina, which was isolated from soil of Kyoto, are rich source of a polyunsaturated fatty acid, arachidonic acid.

Furthermore, we succeeded in the selective production of various polyunsaturated fatty acids, for example, dihomo-γ-linolenic acid and EPA, by controlling of the culture conditions and breeding of the mutant producers. These strains are now under investigation at practical level with 10,000-liter jar fermentor.

We are making further research in microorganisms for the production of novel functional lipids.

 


 

シャーレ上の白いバラ シャーレ上の菌

<Colony formation of filamentous fungi Mortierella>

Genus Mortierella could be classified into two subgenera Mortierella (A) and Micromucor (B)

 

Strains of subgenus Mortierella form rose-like colony on agar plate.

While all the strains of subgenus Mortierella can produce C20 fatty acids, strains of subgenus Micromucor can only produce fatty acids up to C18.

(A) Mortierella alpina 1S-4 (B) Mortierella isabelina CBS 194.28  
シャーレ上の赤いバラ シャーレ上の菌

<Growth of Mortierella fungi on agar plate containing tetrazolium salt.>

 

(A) When M. alpina 1S-4 grew on a agar medium containing triphenyltetrazolium chloride (TCC), oils formed could be stained red.

 

(B) M. isabellina CBS 194.28 grown on the same medium.

(A) Mortierella alpina 1S-4 (B) Mortierella isabelina CBS 194.28  

菌糸の写真<Oil drops accumulated in the cells of M. alpina 1S-4>

 

A large number of oil drops could be observed

 

 

 

 

Finding of mutant that secretes oil outside fungus body

We newly discovered the phenomenon that the mutant secreted a large amount of oil.

シャーレの写真 菌糸の写真

1S-4 = Mortierella alpina 1S-4

V6 = mutant of 1S-4

菌糸の写真

 

 

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Contents ⇒

Bioprocess design/Biofuel production/Bioremediation/Probiotics development/Functional food production